Genexpert Test Gives Early Diagnosis in Early Tuberculosis

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GeneXpert test for Covid

 Gene Xpert Assay is a brand new technology that is rapidly revolutionizing the healthcare industry with its grateful contribution to the early detection of TB or Tuberculosis and drug resistance. TB is a serious threat to public health and society.

What is special about this new technology is that it can help diagnose the presence of mycobacterium tuberculosis in the sputum of a person suspected of having this infection within 2 hours compared to an acid-fast smear test that consumes about 2-8 weeks before an appropriate result will be known. It is also used to test rifampicin resistance.

To Carry your test, search TB test near me center or directly connect with Star Imaging Diagnostic Center. With technological advancements, GeneXpert can also test “covid” patients.

How does Gene Xpert work?

Gene Xpert (Gene Xpert Division for Tuberculosis Elimination, 2016, CDC, March 22, 2021) is a nuclear amplification test in which sputum is removed from a suspected person with Tuberculosis and mixed with a reagent and placed in a disposable tube that works with Gene Xpert, after which other processes are automated.

Find out more about the Genexpert test for covid.

What is the possible result of the GeneXpert test for TB?

The test is expected to show three results – a detectible result, a non-detectable result, and an indeterminate result.

  • Detected signifies that the presence of Mycobacterium Tuberculosis is detected in a sample provided by the patient and tested by GeneXpert.
  • Undetectable means that Mycobacterium Tuberculosis was not found in the sputum probe at the end of the test.
  • Indeterminate means that no result can be determined, so the test still needs to be performed.

In addition, it should be noted that the Gene Xpert test is used to determine rifampicin resistance.

Assume that the individual’s result is positive for Mycobacterium tuberculosis and Rifampicin. This denotes that in this scenario, the bacteria have a high chance of becoming resistant to Rifampicin, for which rapid fasting tests are generally recommended.

Once rifampicin resistance has been confirmed in the test, rapid first- and second-line molecular resistance tests will be performed to determine the best and most effective treatment

However, if the individual result is positive for Mycobacterium tuberculosis but negative for Rifampicin, it means that the bacterium is likely to be easily susceptible to Rifampicin.

GenXpert role for Covid-19 testing

GeneXpert test for Covid is gaining momentum in emergency situations.

We are facing an unusual COVID-19 pandemic with a total of 8,993,659 with 469,587 deaths, according to the latest WHO situation report of June 23, 2020. The various tests available to diagnose COVID-19 infection include RT PCR, isothermal amplification methods, antigen tests, and antibodies. RT-PCR was considered the standard of care for the diagnosis of COVID-19, and sensitivity and specificity data were examined. The positive rate varies according to the sample for analysis; In 205 patients tested by RT-PCR, Positivity values ​​were 93% for bronchoalveolar lavage, 72% for sputum, 63% for nasal swabs, 46% for fiber bronchoscope brush biopsy, and 32% for throat swabs. However, the negative error rate in the COVID-19 studies ranged from 2 to 29%, with sensitivity between 71 and 98%. Various problems with RT-PCR are cost, processing time, and process complexity. RT-PCR testing is recommended for 24 hours for elective surgery in various surgical societies.

According to the Indian Council of Medical Research, high-risk patients undergoing elective surgery should have two negative RT-PCR tests 24 hours apart. It also has the disadvantage of long processing times, which can lead to delays in surgical or life-saving procedures. WHO allows the use of the GeneXpert platform for testing patients with COVID-19. This may mean a difference in testing strategy for COVID-19 in critical situations, such as emergency operations and other life-saving radiological interventions, testing of healthcare professionals, and testing of patients in the ICU. Primary care physicians play an important role in emergency areas at the community level, detecting various diseases; because they are the first contact care healthcare providers.

Primary care physicians can well decide on the urgent requirement for certain interventions, and what tests (RT-PCR vs. GeneXpert) are needed according to the situation in which they are applied. Various benefits include worldwide availability, trained person, short processing time (about 45 minutes), more than 96 tests in 24 hours, half the cost compared to RT-PCR, and use in Africa, Asia, and Latin America. The United States Food and Drug Administration has also granted emergency use permission for the Xpert ®Xpress SARS-CoV-2 test modality. Although GeneXpert sensitivity data for COVID-19 are not available, they contain pathbreaking and reliable data from the tuberculosis sputum test. When used as the first diagnostic test instead of sputum swab microscopy, Xpert MTB / RIF has a sensitivity of 88% and a specificity of 99%.

But, the GeneXpert test for covid has some limitations. The advantage of RT-PCR over GeneXpert is the ability to process a larger number of samples simultaneously. Therefore, RT-PCR is best suited for mass testing, and the use of GeneXpert can save lives in emergency conditions. Although all assays except RT-PCR are not standardized, GeneXpert can be useful for testing the above scenarios. It is used in various hospitals and medical research institutes across India. However, we must keep in mind the problem of testing for Tuberculosis in patients, especially in Africa and Asia, to avoid large-scale GeneXpert testing machines. The Graduate Institute of Medical Education and Research, Chandigarh, India, has started testing GeneXpert in emergency situations such as life-saving radiology procedures and surgery.

Applicability in harsh operating conditions is still a problem

Although GeneXpert is automated and designed for on-site testing, it requires controlled, air-conditioned temperatures, making it unsuitable for adverse field conditions. However, it is widely used in district medical facilities and TB clinics in many low-income countries.

The benefit of GeneXpert is that this test can be much closer to the point of care (instead of just centralized labs), which theoretically can reduce cycle times for test results compared to lab devices,” Source said Health Policy watch.

Finally, instrument-free immunoassays may be cheaper and more suitable for community-level testing. Several quick pipeline tests have taken place but have not yet been approved. Under agreements reached ten years ago, GeneXpert prices, which typically cost approximately $ 17,000, and cartridge prices were reduced for 145 qualified half-and-low income countries. In 2016 alone, the public sector purchased 6.9 million cartridges at concession prices.

To Summarize

  1. GeneXpert is a fast test for COVID-19 with a processing time of about 45 minutes and a low price
  2. Fewer skills are required as already set up for GeneXpert MTB testing in India
  3. It can be life-saving in emergencies because interventions can be made as soon as possible.

Final Words

When you are about to book a TB test near me or geneXpert test for Covid, you must only trust the best in the industry. Star Imaging Diagnostic Center is a reputed name in Delhi for providing best-in-class diagnostic services that are not heavy on your pockets.

You can book a service by visiting the website and arranging for a call-back. You can also send a WhatsApp text message for any queries or book an appointment for one or more diagnostic tests and get accurate test results. 

Book now, and you avail yourself of a good rate for geneXpert test cost in Delhi.

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